On Thursday’s opening day inside the National Stadium, Ashmeade ran the fastest time of 10.07 to take heat 3.
Half-lap specialist Warren Weir followed him home in second place in 10.17, with Chadic Hinds getting third with 10.25.
Training partners Kemar Bailey-Cole and Yohan Blake both clocked 10.26 to go 1-2 in heat 2, with Tyquendo Tracey getting third in 10.29.
Jevaughn Minzie posted a personal best of 10.15 to beat Senoj-Jay Givans, 10.16 and Asafa Powell, 10.17 in heat 1.
Powell, who had a slip at the first time of asking before his heat was recalled, wasn’t too bothered by his third place finish.
“From start to finish I was jogging so it was easy for me,” a confident Powell said.
In the night’s final heat, Bolt started his quest to represent Jamaica at his fourth Olympic Games with a relatively easy 10.15 seconds jog to win.
Everton Clarke finished behind Bolt in 10.16.
In the men’s 400m hurdles, Annsert Whyte (49.37), Isa Phillips and Jaheel Hyde (49.31 both), Romel Lewis 49.66 and Roxroy Cato (49.83) all qualified for the final.
In the women’s section, Ristananna Tracey (55.96) and Kaliese Spencer (56.13) were among the qualifiers for the title race.